Novel bimodal iron oxide particles for efficient tracking of human neural stem cells in vivo. (August 2015)

• Record Type: Journal Article
• Authors: Aswendt, Markus; Henn, Nadine; Michalk, Stefanie; Schneider, Gabriele; Steiner, Mark-Steven; Bissa, Ursula; Dose, Christian; Hoehn, Mathias
• Is Part Of: Nanomedicine. Volume 10:Number 16(2015)
• Page Start: 2499
• Page End: 2512
• Publication Date: 2015-08
• Subjects: bioluminescence imaging -- cell tracking -- iron oxide nanoparticle -- MRI -- neural stem cells; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.15.94
• Languages: English
• ISSNs: 1743-5889

Abstract

Aims: We validated novel bimodal iron oxide particles as substitute of ferumoxides for efficient labeling of human neural stem cells (NSCs). The dextrane-coated FeraTrack Direct (FTD)-Vio particles have additional far-red fluorophores for microscopic cell analysis.Methods: MR relaxometry, spectrophotometric iron determination and microscopy are used for characterization in vitro and in vivo .Results: Efficient uptake is not transfection agent-dependent. FTD-Vio594 labeling had no influence on viability, proliferation, migration and differentiation capacity. It allows MRI-based tracking of engrafted NSCs in mouse brain up to 11 days, complemented by bioluminescence imaging of firefly luciferase expressed by the engrafted cells.Conclusion: Our results highlight the FTD-Vio594 particles as safe and sensitive substitute of ferumoxides for longitudinal tracking of NSCs in preclinical studies.