Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS. (December 2015)

• Record Type: Journal Article
• Authors: Al-Hatmi, Abdullah MS; Normand, Anne-Cécile; van Diepeningen, Anne D; Hendrickx, Marijke; de Hoog, G Sybren; Piarroux, Renaud
• Is Part Of: Future microbiology. Volume 10:Number 12(2015:Dec.)
• Page Start: 1939
• Page End: 1952
• Publication Date: 2015-12
• Subjects: database -- fingerprint -- Fusarium fujikuroi complex -- MALDI-TOF MS -- multi-locus sequence analysis -- spectral analysis; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.15.108
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.Materials & methods : A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.Results: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.Conclusion: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.