Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples. (January 2022)

• Record Type: Journal Article
• Authors: Palát, Jiří; Kukučka, Petr; Codling, Garry P.; Price, Elliott J.; Janků, Petr; Klánová, Jana
• Is Part Of: Chemosphere. Volume 287:Part 3(2022)
• Publication Date: 2022-01
• Subjects: POPs -- NFRs -- Blood serum -- Well-plate SPE -- Chromatography-mass spectrometry; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.132300
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Though many persistent organic pollutants (POPs) are closely regulated the human population is still exposed to these ubiquitous chemicals from the environment and diet. Safe management and human biomonitoring of POPs is necessary to understand the risk of exposure. Within human biomonitoring the mass of sample is often limited, therefore robust methods using smaller sample amounts are necessary. This study developed a 96-well plate solid phase extraction (SPE) method for determination of selected POPs: polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCD) and non-persistent novel flame retardants (NFRs) in low volume blood serum. Non-destructive clean-up coupling Oasis HLB extraction plate with Phree phospholipid removal plate was employed. Extraction efficiency was determined at low and high concentrations in certified reference materials NIST SRM 1957 and 1958, respectively. Target compounds deviated from certified values on average by 15% and 21% for SRM 1957 and SRM 1958, respectively. Observed limit of detections (LODs) ranged from 0.36 pg/mL (PCB 180) to 66.07 pg/mL (δ-HCH). The applicability for real samples is demonstrated on 48 samples from pregnant women enrolled in the pilot phase of the CELSPAC: TNG study. In total, 30 target compounds were detected in at least one sample. The method developed here provides a fast and reliable analysis of human blood serum with possibility to introduce automation for the sample preparation procedure. Highlights: Validated SPE method for blood serum analysis of selected POPs was developed. Method uses low volumes of blood serum (200–250 μL). Use of 96 well plate format allows future automation of sample preparation. Method is applicable to large scale human biomonitoring.