Novel enzymatic graphene oxide based biosensor for the detection of glutathione in biological body fluids. (January 2022)

• Record Type: Journal Article
• Authors: Cheraghi, Somaye; Taher, Mohammad A.; Karimi-Maleh, H.; Karimi, Fatmeh; Shabani-Nooshabadi, Mehdi; Alizadeh, Marzieh; Al-Othman, Amani; Erk, Nevin; Yegya Raman, Praveen Kumar; Karaman, Ceren
• Is Part Of: Chemosphere. Volume 287:Part 2(2022)
• Publication Date: 2022-01
• Subjects: Glutathione peroxidase -- Enzymatic biosensor -- Glutathione -- Graphene oxide -- Nafion -- Voltammetric method; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.132187
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In this work, we report a novel enzymatic biosensor based on glutathione peroxidase (GSH-Px), graphene oxide (GO) and nafion for the electrochemical sensing of glutathione (GSH) in body fluids. GSH-Px was immobilized covalently via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) onto modified glassy carbon electrode (GCE) decorated with GO and nafion and successfully used for sensing of GSH in the presence of H2 O2 as catalyst with Michaelis-Menten constant about 0.131 mmol/L. The active surface are of GCE improve from 0.183 cm 2 to 0.225 cm 2 after modification with GO. The introduced biosensor (GSH-Px/GO/nafion/GCE) was used for monitoring of GSH over the range 0.003–370.0 μM, with a detection limit of 1.5 nM using differential pulse voltammetric (DPV) method. The GSH-Px/GO/nafion/GCE was successfully applied to the determination of GSH in real samples. Highlights: First nanostructure enzymatic biosensor for determination of glutathione. Pharmaceutical and biological glutathione sensing. Simple and selective strategies for bio-sensing of glutathione.