3D‐Printed Reinforcement Scaffolds with Targeted Biodegradation Properties for the Tissue Engineering of Articular Cartilage. Issue 23 (18th October 2021)

• Record Type: Journal Article
• Authors: Tosoratti, Enrico; Fisch, Philipp; Taylor, Scott; Laurent‐Applegate, Lee Ann; Zenobi‐Wong, Marcy
• Is Part Of: Advanced healthcare materials. Volume 10:Issue 23(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-18
• Subjects: 3D‐printing -- cartilage engineering -- enzymatically crosslinked hydrogels -- hybrid reinforcement scaffolds -- lactide‐copolymers; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202101094
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Achieving regeneration of articular cartilage is challenging due to the low healing capacity of the tissue. Appropriate selection of cell source, hydrogel, and scaffold materials are critical to obtain good integration and long‐term stability of implants in native tissues. Specifically, biomechanical stability and in vivo integration can be improved if the rate of degradation of the scaffold material matches the stiffening of the sample by extracellular matrix secretion of the encapsulated cells. To this end, a novel 3D‐printed lactide copolymer is presented as a reinforcement scaffold for an enzymatically crosslinked hyaluronic acid hydrogel. In this system, the biodegradable properties of the reinforced scaffold are matched to the matrix deposition of articular chondrocytes embedded in the hydrogel. The lactide reinforcement provides stability to the soft hydrogel in the early stages, allowing the composite to be directly implanted in vivo with no need for a preculture period. Compared to pure cellular hydrogels, maturation and matrix secretion remain unaffected by the reinforced scaffold. Furthermore, excellent biocompatibility and production of glycosaminoglycans and collagens are observed at all timepoints. Finally, in vivo subcutaneous implantation in nude mice shows cartilage‐like tissue maturation, indicating the possibility for the use of these composite materials in one‐step surgical procedures. Abstract : The biodegradable properties of a novel lactide copolymer are matched to the matrix deposition of articular chondrocytes embedded in an enzymatically crosslinked hyaluronic acid‐based hydrogel. The lactide reinforcement provides stability to the soft hydrogel in the early stages and degrades once sufficient matrix has been deposited by cells. Excellent biocompatibility and production of glycosaminoglycans and collagens are observed at all timepoints.