Isopropanol-butanol-ethanol production by cell-immobilized vacuum fermentation. (January 2022)

• Record Type: Journal Article
• Authors: Ferreira dos Santos Vieira, Carla; Duzi Sia, Augusto; Maugeri Filho, Francisco; Maciel Filho, Rubens; Pinto Mariano, Adriano
• Is Part Of: Bioresource technology. Volume 344:Part B(2022)
• Publication Date: 2022-01
• Subjects: Butanol -- IBE fermentation -- Repeated batch -- In-situ vacuum recovery; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126313
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Clostridium beijerinckii DSM 6423 was cultivated under vacuum in repeated batches. The cell carrier (sugarcane bagasse) was packed in concentric annular baskets. Glucose (∼60 g/L) was consumed by up to 96% in one of the batch cycles. The IBE productivity was 0.35 g/L∙h on average during three cycles. The concept of an internal-loop boiling-driven fibrous-bed bioreactor was developed. Abstract: The Isopropanol-Butanol-Ethanol productivity by solventogenic clostridia can increase when cells are immobilized on low-cost, renewable fibrous materials; however, butanol inhibition imposes the need for dilute sugar solutions (less than40 g/L). To alleviate this problem, the in-situ vacuum product recovery technique was applied to recover IBE in repeated-batch cultivation of Clostridium beijerinckii DSM 6423 immobilized on sugarcane bagasse. Five repeated batch cycles were conducted in a 7-L bioreactor containing P2 medium (∼60 g/L glucose) and bagasse packed in 3D-printed concentric annular baskets. In three cycles, glucose was consumed by 86% on average, the IBE productivity was 0.35 g/L∙h or 30% and 17% higher relative to free- and immobilized (without vacuum)-cell cultures. Notably, the product stream contained 45 g/L IBE. However, the fermentation was unsatisfactory in two cycles. Finally, by inserting a fibrous bed with hollow annuli in a vacuum fermentation, this work introduces the concept of an internal-loop boiling-driven fibrous-bed bioreactor.