Copper ions binding regulation for the high-efficiency biodegradation of ciprofloxacin and tetracycline-HCl by low-cost permeabilized-cells. (January 2022)

• Record Type: Journal Article
• Authors: Zhou, Yu; You, Shengping; Zhang, Jiaxing; Wu, Min; Yan, Xiaohui; Zhang, Chengyu; Liu, Yuxuan; Qi, Wei; Su, Rongxin; He, Zhimin
• Is Part Of: Bioresource technology. Volume 344:Part B(2022)
• Publication Date: 2022-01
• Subjects: Laccase -- Antibiotics -- Permeabilized cells -- Molecular docking; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126297
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The regulation of Cu 2+ binding time significantly improves the activity of laccase. The low-cost permeabilized-cells were prepared to efficiently degrade antibiotics. The biodegradation mechanism and pathways of CIP and TCH were analyzed. Abstract: Cu 2+ plays a decisive role for the bio-oxidation in the active center of laccase. In the fermentation-purified process, the loss of Cu 2+ reduces the activity and the high cost limits the application of laccase. In this study, a fermentation-permeabilization combined process were developed which based on the regulation of Cu 2+ binding time to produce the permeabilized-cells containing laccase, in which Cu 2+ can enter the cells freely to greatly improve the laccase activity and reduce the immobilization cost by about 19 times. So, the permeabilized-cells is suitable for biodegradation of antibiotic pollution in the environment, which was applied for the biodegradation of ciprofloxacin (CIP) and tetracycline-HCl (TCH) and the degradation efficiency reached 95.42% and 98.73%, respectively, with low ecotoxicity of the degradation products. Finally, the degradation mechanism was analyzed theoretically by molecular docking. Therefore, this study provided a low-cost, eco-friendly, and widely applicable method for organic pollutants removal.