Sodium chloride stimulates the biomass and astaxanthin production by Haematococcus pluvialis via a two-stage cultivation strategy. (January 2022)

• Record Type: Journal Article
• Authors: Li, Qingqing; You, Jinkun; Qiao, Tengsheng; Zhong, Du-bo; Yu, Xuya
• Is Part Of: Bioresource technology. Volume 344:Part A(2022)
• Publication Date: 2022-01
• Subjects: Haematococcus pluvialis -- Biomass -- Astaxanthin -- NaCl -- Two-stage cultivation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126214
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A two-stage strategy using NaCl for algal biomass and astaxanthin yield was proposed. Low-dose NaCl induced algal growth, while the high-dose facilitated astaxanthin synthesis. Sophisticated crosstalk occurred among ROS, GABA and Ca 2+ in astaxanthin accumulation. Abstract: A major challenge facing by astaxanthin industrialization is the low productivity and high production costs. This study established a two-stage cultivation strategy based on the application of NaCl to improve the production of biomass and astaxanthin by Haematococcus pluvialis. During the first growth stage, 12.5 mg L -1 NaCl led to a remarkable enhancement in biomass, which was 1.28 times compared with the control. Moreover, 2 g L -1 NaCl stimulated the astaxanthin content from 12.18 mg g −1 to 25.92 mg g −1 during the second induction stage. Simultaneously, salinity stress application increased the lipids and GABA contents, as well as the levels of Ca 2+ and carotenogenic genes' expression, but suppressed the contents of carbohydrate and protein and high-light induced-ROS. This study proposed a simple and convenient strategy for efficient coproduction of biomass and astaxanthin and provides insights into the underlying mechanism of astaxanthin biosynthesis in H. pluvialis induced by salinity stress.