Laminaria japonica hydrolysate promotes fucoxanthin accumulation in Phaeodactylum tricornutum. (January 2022)

• Record Type: Journal Article
• Authors: Wang, Zhi-Peng; Wang, Pei-Kang; Ma, Yan; Lin, Jia-Xue; Wang, Cheng-Long; Zhao, Yu-Xiang; Zhang, Xin-Yue; Huang, Bei-Chen; Zhao, Shou-Geng; Gao, Lei; Jiang, Jing; Wang, Hai-Ying; Chen, Wei
• Is Part Of: Bioresource technology. Volume 344:Part A(2022)
• Publication Date: 2022-01
• Subjects: Fucoxanthin -- Phaeodactylum tricornutum -- Autotrophic culture -- Laminaria japonica -- Plant hormones; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126117
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: LPH promote fucoxanthin accumulation and growth in P. tricornutum simultaneously. Plant hormones were found to promote fucoxanthin accumulation for the first time. Genes related to fucoxanthin formation were generally up-regulated. Abstract: Fucoxanthin (Fx) has gained a growing attention due to the remarkable biological activities. The limited biomass of was the restrictive factor for Fx production in Phaeodactylum tricornutum . In this study, Laminaria japonica hydrolysate (LPH) with a low addition proportion of 1.5 ml/L, was proved to promote fucoxanthin accumulation and cell growth simultaneously. Fx topped at 27.9 mg/L after 10-d cultivation in the LPH group, with a biomass of 1.59 g/L and a Fx content of 17.55 mg/g. Three key plant hormones in LPH were screened responsible for promoting fucoxanthin accumulation. Transcriptomic analysis and qRT-PCR results showed that genes related to Fx formation were generally up- regulated. The study demonstrated that LPH addition was a feasible and efficient strategy to enhance production of fucoxanthin, facilitating the scale-up production of Fx in autotrophic culture.