Biochemical defense responses of tolerant and susceptible lettuce accessions following infection by Sclerotinia sclerotiorum. (27th August 2021)

• Record Type: Journal Article
• Authors: Almatwari, Aqeel Hussein Abdulraoof; Hassandokht, Mohammadreza; Soltani, Frouzandeh; Mirzadi Gohari, Amir; Javan-Nikkhah, Mohammad
• Is Part Of: Archives of phytopathology and plant protection. Volume 54:Number 13/14(2021)
• Page Start: 903
• Page End: 917
• Publication Date: 2021-08-27
• Subjects: Biochemical markers -- Lipid peroxidation -- Lettuce and Sclerotinia sclerotiorum; Plants, Protection of -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1080/03235408.2020.1869385
• Languages: English
• ISSNs: 0323-5408

Abstract

Abstract: Sclerotinia sclerotiorum is one of the most destructive lettuce diseases, restricting lettuce cultivation globally. Here, we measured the activity of three antioxidant enzymes in two contrastingly responding lettuce accessions (susceptible ML1 and tolerant Jah accessions) challenged by S. sclerotiorum . These enzymes included superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT). Moreover, we applied the 2, 2-diphenylpicrylhydrazyl (DPPH) method and the malondialdehyde (MDA) assay. The activities of SOD and GPx were much pronounced in the tolerant accession than in the susceptible one. Our results indicate that MDA concentration increased significantly in the leaves of tolerant accession compared with that of the susceptible one at 1 dpi whereas MDA level was pronounced in the later time points in the susceptible accession compared with tolerant accession. Taken together, we observed that the examined biochemical markers differentially fluctuated in the applied accessions, shedding light to understand better resistance mechanisms involved in restricting this notorious fungal pathogen.