Isolation of a new Streptomyces virginiae W18 against fish pathogens and its effect on disease resistance mechanism of Carassius auratus. (December 2021)

• Record Type: Journal Article
• Authors: Hu, Wenbin; Yu, Xiaojing; Jin, Duo; Zhai, Feng; Zhou, Pengji; Ali, Khan Tahir; Cui, Jun; Wang, Pan; Liu, Xiong; Sun, Yunjun; Yi, Ganfeng; Xia, Liqiu
• Is Part Of: Microbial pathogenesis. Volume 161:Part A(2021)
• Publication Date: 2021-12
• Subjects: Antagonistic bacteria -- Fish pathogens -- Carassius auratus -- Immune response -- Genome sequencing; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2021.105273
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The Streptomyces virginiae strain W18 was screened from soil, which exhibited broad-spectrum antibacterial activity against fish pathogens. Safety assays showed that strain W18 had no toxicity to fish. Additionally, strain W18 promoted the growth performance of Carassius auratus after feeding in feed mixed with bacteria for one month. Moreover, the activities of AKP, ACP, and SOD in the serum of C. auratus were significantly increased, while the activity of LZM did not greatly change. To detect the expression levels of the genes related to immune factors in the livers, kidneys, and spleens of C. auratus, qRT-PCR was performed. The expression levels of KEAP1, IL-8, TNF-α, IL-β, and C3 were upregulated in all three organs compared to the control, but LZM expression was downregulated in the kidney. The challenge experiment illustrated that the probability of infection with Aeromonas veronii was reduced by 60% and 40% when C. auratus was fed with two different doses of strain W18 in advance. The whole genome of strain W18 was sequenced, and the gene clusters of secondary metabolites in strain W18 were analyzed by AntiSMASH. The results showed that strain W18 contained a total of 26 gene clusters, and functional annotation analysis was conducted by using the non-coding databases COG and KEGG. All of the above results indicated that the use of strain W18 as a feed additive could enhance the resistance of C. auratus toward pathogenic bacteria and disease. In conclusion, an antagonistic strain (W18) against fish pathogenic bacteria was obtained in this study, which is of great significance for finding new treatment methods for bacterial diseases in the aquaculture industry. Highlights: A new Streptomyces virginiae strain W18 was isolated and it showed broad-spectrum bacteriostatic effect against fish pathogens. For the first time, it was found that strain W18 could improve the immunity and growth performance of C. auratus, and also enhance the resistance of C. auratus to Aeromonas veronii. Strain W18 contained a large number of gene clusters related to antimicrobial activity as revealed by genomic analysis.