The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF-κB Signaling Pathway. (16th November 2021)

• Record Type: Journal Article
• Authors: Jiang, Siyi; Wei, Qiong; Ye, Xiaochuan; Luo, Dan; Zhang, Xiaoyan; Li, Zhenglei; You, Pengtao; Huang, Xianzhang; Liu, Yanwen
• Other Names: Haque Areeful Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2021(2021)
• Publication Date: 2021-11-16
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2021/9958808
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Background . Traditional Chinese medicine Smilax is the rhizome of liliaceous plant Smilax china L., which is used to treat pelvic inflammatory disease and anxieties. Purpose . To investigate the mechanism of anti-inflammatory activity of the extract from Smilax china L. (ES). Methods . The components of ES were identified by UPLC-QTOF-MS/MS. The anti-inflammatory activities were evaluated in xylene-induced ear oedema and egg white-induced plantar swelling test. Cell viability was examined by CCK-8 assay. The inflammatory mediators, proinflammatory cytokines, and MAPK and NF- κ B signals in LPS-stimulated THP-1 cells were determined using ELISA, real-time PCR, and Western blot, respectively. Results . 20 compounds of ES were confirmed by comparing with the reference substance. ES displayed more prominent anti-inflammatory activity than the positive control "Jin Gang Teng" capsule in the in vivo acute inflammatory model. ES suppressed the expression of PGE2 and 6-Keot-PGF1 α, and the ratio of IC50 (COX-1)/IC50 (COX-2) of ES was 3.15, which indicated that ES could selectively inhibit COX-2. ES dose-dependently (12.5, 25, and 50 mg/L) decreased the production and mRNA levels of proinflammatory cytokines IL-1 β, IL-6, and TNF- α . Furthermore, ES significantly decreased LPS-induced phosphorylation of p38, JNK, ERK1/2, and p65, inhibiting the expression of IKK α and the degradation of I κ B α . Conclusion . The results suggested that ES could selectively inhibit the activity of COX-2, and the anti-inflammatory effect of ES was associated with the inhibition of IL-1 β, IL-6, and TNF- α via negative regulation of MAPK and NF- κ B signaling pathways in LPS-induced THP-1 cells.