3D bioprinting of multicellular scaffolds for osteochondral regeneration. (October 2021)

• Record Type: Journal Article
• Authors: Qin, Chen; Ma, Jingge; Chen, Lei; Ma, Hongshi; Zhuang, Hui; Zhang, Meng; Huan, Zhiguang; Chang, Jiang; Ma, Nan; Wu, Chengtie
• Is Part Of: Materials today. Volume 49(2021)
• Page Start: 68
• Page End: 84
• Publication Date: 2021-10
• Subjects: Bioprinting -- Bioink -- Bioceramic -- Osteochondral tissues; Materials science -- Periodicals; Metallurgy -- Periodicals; Metal-work -- Periodicals; Biomedical and Dental Materials -- Periodicals; Manufactured Materials -- Periodicals; Metals -- Periodicals; 620.11
• DOI: 10.1016/j.mattod.2021.04.016
• Languages: English
• ISSNs: 1369-7021

Abstract

Graphical abstract: Abstract: Regeneration of osteochondral tissue is of great potentialities in repairing severe osteochondral defects. However, anisotropic physiological characteristics and tissue linage difference make the regeneration of osteochondral tissue remain a huge challenge. Herein, a multicellular system based on a bilayered co-culture scaffold mimicking osteochondral tissues was successfully developed for an alternative of osteochondral regeneration via a 3D bioprinting strategy. The dual function of integrally repairing both cartilage and bone could be achieved by designing multiple-cells distribution and a cell-induced bioink containing bioceramic particles. As an important bioactive agent, the Li-Mg-Si bioceramics-containing bioink exhibited the function of simultaneously stimulating multiple cells for differentiation towards specific directions. The 3D bioprinted co-culture scaffolds showed the capacity for osteochondral tissue regeneration by inducing osteogenic and chondrogenic differentiation in vitro and accelerating the repair of severe osteochondral defects in vivo . This study offers a potential strategy for complex tissue reconstruction through bioprinting multiple tissue cells in combination of bioceramics-stimulating bioinks.