LINC00853 restrains T cell acute lymphoblastic leukemia invasion and infiltration by regulating CCR9/CCL25. (December 2021)

• Record Type: Journal Article
• Authors: Li, Jingyuan; Muhammad, Jamal; Xie, Tian; Sun, Jiaxing; Lei, Yufei; Wei, Zimeng; Pan, Shan; Qin, Hong; Shao, Liang; Jiang, Daozi; Zhang, Qiuping
• Is Part Of: Molecular immunology. Volume 140(2021)
• Page Start: 267
• Page End: 275
• Publication Date: 2021-12
• Subjects: T-ALL T cell acute lymphoblastic leukemia -- lncRNA long non-coding RNA -- CCR9 CC chemokine receptor 9 -- CCL25 CC chemokine ligand 25 -- GEO gene expression omnibus -- GFP green fluorescence protein -- NOD/SCID nonobese diabetic/severe combined immune deficien -- BM bone marrow -- PBMCs peripheral blood mononuclear cells -- AML acute myelocytic leukemia -- B-ALL B cell acute lymphoblastic leukemia -- CML chronic myelocytic leukemia -- CLL chronic lymphocytic leukemia -- MDS myelodysplastic syndrome; T-ALL -- LINC00853 -- CCR9 -- CCL25 -- Invasion -- Infiltration; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2021.10.016
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: This is the first study deciphering the relationship of LINC00853 and CCR9, we speculate that LINC00853 regulates the CCR9 modulating the binding of CCR9 and CCL25. These findings provide new possibilities in understanding the relationship between lncRNAs and chemokines. This study may present a new research direction of therapeutic targeting of these lncRNAs to restrain in T-ALL relapse. Abstract: Background: Leukemia is a group of hematopoietic malignancies characterized by the accumulation and infiltration of abnormal hematopoietic stem cells or early progenitor cells. T cell acute lymphoblastic leukemia (T-ALL) is a hematologic malignancy occurring in 15 % of pediatric and 25 % of adult ALL cases. Infiltration and metastasis of leukemic cells to specific organs are consequences of disease relapse and dismal prognosis. Long non-coding RNAs (lncRNAs) have been identified to function in the migration, invasion and infiltration of tumors by regulating gene expression. Our previous studies showed that CC chemokine receptor 9 (CCR9), which specifically bind to CC chemokine ligand 25 (CCL25), promotes T-ALL infiltration. Methods: Bioinformatic methods were used to screen LINC00853 in gene expression omnibus (GEO) datasets. RT-qPCR, western bolt and flow cytometry were applied to detect the expression of LINC00853 and CCR9. Transwell and martrigel-transwell were employed to assess the cells migration and invasion abilities. Fluorescence microscope was applied to observed the green fluorescence protein positive (GFP + ) cells. Lentivirus and adenovirus were packed to construct nc-blank, sh-LINC00853-blank and sh-LINC00853-rescue jurkat cell lines. Results: In this study, we found out the negative correlation of LINC00853 and CCR9 expression. LINC00853 was downregulated while CCR9 was upregulated in GEO datasets, T-ALL cell lines and clinical samples. Moreover, LINC00853 suppressed jurkat cells migration and invasion in vitro and restrained infiltration in liver, spleen, kidney, lung, brain, ovary of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Conclusions: These findings indicate that LINC00853 restrains T-ALL cell invasion and infiltration by regulating CCR9/CCL25.