Self-resetting molecular probes for nucleic acids detection enabled by fuel dissipative systems. (December 2021)

• Record Type: Journal Article
• Authors: Li, Na; Zhao, Yuee; Liu, Yu; Yin, Zhe; Liu, Rui; Zhang, Linghao; Ma, Liang; Dai, Xiaochuan; Zhou, Dongsheng; Su, Xin
• Is Part Of: Nano today. Volume 41(2021)
• Publication Date: 2021-12
• Subjects: COVID-19 -- SARS-CoV-2 -- DNA nanotechnology -- Fuel dissipation -- Kinetic simulation -- MD simulation -- Toehold mediated strand displacement -- Exonuclease III; Nanotechnology -- Periodicals; Nanosciences -- Périodiques; 620.505
• DOI: 10.1016/j.nantod.2021.101308
• Languages: English
• ISSNs: 1748-0132

Abstract

Highlights: Self-resetting probe based on dynamic DNA nanotechnology is used for repeated SARS-CoV-2 RNA detection. Highly consistent signal across cyclic detections and short cycling time were achieved by utilizing simulation tools. The featured dissipative kinetics of the probe enables a fingerprint-type FWHM to identify single-nucleotide variants. This method was deployed for COVID-19 clinical samples and 100% correctly classify patients and controls. Graphical Abstract: ga1 Abstract: A once-in-a-century global public health crisis, the COVID-19 pandemic has damaged human health and world economy greatly. To help combat the virus, we report a self-resetting molecular probe capable of repeatedly detecting SARS-CoV-2 RNA, developed by orchestrating a fuel dissipative system via DNA nanotechnology. A set of simulation toolkits was utilized to design the probe, permitting highly consistent signal amplitudes across cyclic detections. Uniquely, full width at half maximum regulated by dissipative kinetics exhibits a fingerprint signal suitable for high confidential identifications of single-nucleotide variants. Further examination on multiple human-infectious RNA viruses, including ZIKV, MERS-CoV, and SARS-CoV, demonstrates the generic detection capability and superior orthogonality of the probe. It also correctly classified all the clinical samples from 55 COVID-19 patients and 55 controls. Greatly enhancing the screening capability for COVID-19 and other infectious diseases, this probe could help with disease control and build a broader global public health agenda.