801 EXOSOMES CONTAINING LUNG SELF-ANTIGENS COLLAGEN-V AND Kα1-TUBULIN ARE PREVALENT IN PATIENTS WITH SYMPTOMATIC GASTROESOPHAGEAL REFLUX: RESULTS FROM A PILOT STUDY. (17th September 2021)

• Record Type: Journal Article
• Authors: Razia, Deepika; Ravichandran, Ranjithkumar; Bansal, Sandhya; Fournier, Sarah; Baboli, Komeil; Bremner, Ross M; Mohanakumar, Thalachallour; Mittal, Sumeet K
• Is Part Of: Diseases of the esophagus. Volume 34(2021)Supplement 1
• Publication Date: 2021-09-17
• Subjects: Esophagus -- Diseases -- Periodicals; 616.32
• DOI: 10.1093/dote/doab052.801
• Languages: English
• ISSNs: 1120-8694

Abstract

Abstract: : Gastroesophageal reflux disease (GERD) has been associated with aspiration-induced pulmonary injury; however, good clinical or laboratory markers are not available. Increased serum levels of exosomes containing normally sequestered primary lung self-antigens (collagen-V, Kα1-tubulin) have been associated with lung injury in the lung transplant population. The aim of this pilot study was to assess the prevalence of exosomes containing collagen-V and/or Kα1-tubulin in patients with severe GERD. Methods: After IRB approval, the institutional biobank database was queried to identify non-lung transplant patients who underwent primary anti-reflux surgery (ARS) from 2019 to 2020. Serum samples were retrieved from the repository. Exosome pellets were isolated using the Invitrogen® kit using the manufacturer's protocol. The size of exosomes in the pellet was confirmed using NanoSight. Western blot of the exosomes was used to isolate and quantify collagen-V and Kα1-tubulin, using CD-9 as the standard. A ratio > 1 was considered abnormal. Results: Ten patients (6 females) with a median (IQR) age of 53 (42, 63) years were included in this study. All patients had symptomatic GERD as an indication for ARS. Five patients (50%) had exosomes containing abnormal levels of collagen-V and/or Kα1-tubulin (Figure 1). There was a mean 2.9- and 8.2-fold increase in collagen-V and Kα1-tubulin, respectively. Conclusion: Humoral factors associated with lung injury are highly prevalent in patients undergoing elective ARS for GERD. This suggests that detection of exosomes containing lung self-antigens collagen-V and Kα1-tubulin could be useful as a biomarker of GERD-induced lung injury.