Metanil yellow promotes oxidative stress, astrogliosis, and apoptosis in the cerebellar cortex of adult male rat with possible protective effect of scutellarin: A histological and immunohistochemical study. (December 2021)

• Record Type: Journal Article
• Authors: Tawfeek, Shereen Elsayed; Shalaby, Amany Mohamed; Alabiad, Mohamed Ali; Albackoosh, Al-Amari Ali Almbruk; Albakoush, Khalid Mohammed Mohammed; Omira, Mahmoud Mohamad Ahmad
• Is Part Of: Tissue & cell. Volume 73(2021)
• Publication Date: 2021-12
• Subjects: Cerebellum -- Metanil yellow -- Scutellarin -- GFAP -- Synaptophysin -- Cleaved caspase-3; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2021.101624
• Languages: English
• ISSNs: 0040-8166

Abstract

Graphical abstract: Highlights: Metanil yellow is a food dye that has harmful impacts on different body systems. Chronic administration of metanil yellow causes alterations in the cerebellar cortex of rats. Scutellarin has neuroprotective activity through its antioxidant and antiapoptotic effects. Scutellarin could protect against metanil yellow-induced alterations by reducing oxidative stress and minimizing gliosis. Abstract: Metanil yellow is a food dye that has harmful impacts on different body systems. Scutellarin has antioxidant, antiapoptotic, and anti-inflammatory activities. The aim of the current research was to study the effect of chronic administration of metanil yellow on the cerebellar cortex of rats and to evaluate the protective effect of scutellarin. Forty adult male rats were allocated into four groups: group I acted as control, group II was administrated scutellarin (100 mg/kg/day), group III was administrated metanil yellow (200 mg/kg/day), and group IV was administrated scutellarin and metanil yellow as in group II and group III. The agents were administered via oral gavage for 8 weeks. Metanil yellow induced a significant rise in the malondialdehyde coupled with a significant reduction in the superoxide dismutase and glutathione peroxidase. The Purkinje cells were irregular and shrunken with condensed nuclei. A significant elevation in glial fibrillary acidic protein (GFAP) and cleaved caspase-3 as well as a significant reduction of synaptophysin expression were revealed in comparison with the control group. Interestingly, few changes were noticed in rats given metanil yellow concomitant with scutellarin. In conclusion, scutellarin could protect against metanil yellow-induced alterations in the cerebellar cortex by reducing oxidative stress and minimizing gliosis.