FRI0025 CIRCADIAN RHYTHMS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE PRODUCTION IN FEMALES WITH RHEUMATOID ARTHRITIS AND ARTERIAL HYPERTENSION DEPENDING ON NOS3 Т786С GENE POLYMORPHISM. (13th June 2020)

• Record Type: Journal Article
• Authors: Zaichko, K.; Stanislavchuk, M.; Zaichko, N.; Khomenko, V.
• Is Part Of: Annals of the rheumatic diseases. Volume 79(2020)Supplement 1
• Page Start: 585
• Page End: 585
• Publication Date: 2020-06-13
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2020-eular.4254
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: One of the most common comorbidity in patients with rheumatoid arthritis (RA) is arterial hypertension (AH), with incidence ranging from 20 to 60%. Mechanisms of this comorbidity arises a lot of interest. In our previous study was established the association of T786C NOS3 (rs2070744) gene polymorphism with AH in females with RA in the Ukrainian population [1]. Objectives: So next, we were aiming to investigate daily fluctuation of endothelial nitric oxide synthase (NOS3) in RA patients with AH depending on NOS3 T786C gene polymorphism. Methods: In the study were enrolled 173 females with RA aged 43.7 ± 7.35 years (Mean ± SD) and 34 age-matched healthy women without joint diseases and autoimmune diseases (control). Serum NOS3 level was determined at 08:00 and 20:00 using Cloud-Clone Corp kits (USA). NOS3 T-786С polymorphism was determined by Real-Time PCR (Bio-Rad iCycler IQ5) using SNP-express kit. Study was carried out in compliance with bioethical standards and provisions of the WHO, Helsinki Declaration of the General Assembly of the World Medical Association (1989). Results: Among enrolled patients prevailed individuals with more than 5 years disease history, ІІ-ІІІ radiographic stage (80.9 %), and were seropositive for anti-cyclic citrullinated peptide (80.6%). There were 114 (66%) normotensive patients and 59 (34%) patients with AH (13% - I stage, 20.8% - II stage). The daily fluctuation of NOS3 serum level was established in the control group. The evening NOS3 level was higher in 1.3 times, than the morning level (р<0.001). In RA patients the similar fluctuations of NOS3 level was registered, but the daily NOS3 production was lower, than in control. Diurnal variation of NOS3 level depended on comorbid AH and NOS3 T-786С genotype. In CC genotype NOS3 levels at 08:00 and at 20:00 were lower in 1.2-1.3 times (p<0.05) than in TT and TC genotypes. In patients with RA and AH the lowest diurnal variation of NOS3 level was in CC genotype. The decrease of evening NOS3 production was strong associated with comorbid AH (OR 3.78; 95% CI 1.96-7.28). Conclusion: Circadian rhythms of NOS3 production in females with RA and AH depend on NOS3 Т786С gene polymorphism. The depression of NOS3 production in the evening can be predictor of comorbid AH in females with RA. References: [1]Zaichko, K., & Stanislavchuk, M. (2017, April). T-786C gene promoter polymorphism of endothelial nitric oxide synthase: its association to the disease activity and arterial hypertension in patients with rheumatoid arthritis in the ukraine population. In rheumatology (vol. 56, pp. 129-129). GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS. Acknowledgments: NA Disclosure of Interests: None declared