Unraveling the unique butyrate re-assimilation mechanism of Clostridium sp. strain WK and the application of butanol production from red seaweed Gelidium amansii through a distinct acidolytic pretreatment. (December 2021)

• Record Type: Journal Article
• Authors: Zhang, Kan; Hong, Ying; Chen, Chaoyang; Wu, Yi-Rui
• Is Part Of: Bioresource technology. Volume 342(2021)
• Publication Date: 2021-12
• Subjects: Re-assimilation -- Metabolic analysis -- Butanol -- Algal biomass -- Hydrolysis -- Solventogenic clostridia; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125939
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Clostridium sp. strain WK demonstrates a strong tolerance of butyrate up to 30 g/L. The butanol production was enhanced to be 13.96 g/L by adding 24 g/L butyrate. Genes buk and ptb were more crucial than ctfAB for the butyrate re-assimilation. An improved yield of butanol from Gelidium amansii was established by 29.9 folds. Abstract: Exploration of the algae-derived biobutanol synthesis has become one of the hotspots due to its highly cost-effective and environment-friendly features. In this study, a solventogenic strain Clostridium sp. strain WK produced 13.96 g/L butanol with a maximal yield of 0.41 g/g from glucose in the presence of 24 g/L butyrate. Transcriptional analysis indicated that the acid re-assimilation of this strain was predominantly regulated by genes buk - ptb rather than ctfAB, explaining its special phenotypes including high butyrate tolerance and the pH-independent fermentation. In addition, a butyric acid-mediated hydrolytic system was established for the first time to release a maximal yield of 0.35 g/g reducing sugars from the red algal biomass ( Gelidium amansii ). Moreover, 4.48 g/L of butanol was finally achieved with a significant enhancement by 29.9 folds. This work reveals an unconventional metabolic pathway for butanol synthesis in strain WK, and demonstrates the feasibility to develop renewable biofuels from marine resources.