Yeast culture supplementation alters the performance and health status of juvenile largemouth bass (Micropterus salmoides) fed a high‐plant protein diet. (2nd November 2021)

• Record Type: Journal Article
• Authors: Xv, Zhicheng; Zhong, Yunfei; Wei, Yexin; Zhang, Tingting; Zhou, Wenhao; Jiang, Yanzhe; Chen, Yongjun; Lin, Shimei
• Is Part Of: Aquaculture nutrition. Volume 27:Number 6(2021)
• Page Start: 2637
• Page End: 2650
• Publication Date: 2021-11-02
• Subjects: antioxidant capacity -- cottonseed protein concentrate -- intestinal microbiota -- Micropterus salmoides -- yeast culture; Aquaculture -- Periodicals; Aquatic animals -- Feeding and feeds -- Periodicals; Fishes -- Feeding and feeds -- Periodicals; 639.3
• DOI: 10.1111/anu.13391
• Languages: English
• ISSNs: 1353-5773

Abstract

Abstract: The present study was conducted to investigate the effects of yeast culture on the growth, hepatic antioxidant capacity, intestinal barrier and microflora of fish fed a high‐cottonseed protein diet. The largemouth bass (initial body weight: 12.17 ± 0.02 g) were randomly allotted into three treatments, fish meal‐based diet (FM), cottonseed protein concentrate‐based diet (CPC, 36% of the fish meal replaced by cottonseed protein concentrate) and CPC diet with yeast culture (CPCY, CPC+3% yeast culture) for 8 weeks, respectively. The results indicated fish fed 3% yeast culture diet could enhance specific growth rate (SGR), protein efficiency ratio (PER) and feeding rate (FR), while the hepatosomatic index (HSI) and liver glycogen content were significantly decreased compared with the CPC group ( p < .05). Compared with the FM group, the CPC diet resulted in liver tissue damage accompanied by upregulation of pro‐inflammatory factor ( IL ‐ 1β and TNFα ) expression, while adding yeast culture significantly increased the relative expression of liver anti‐inflammatory factor ( IL ‐ 10 ) and antioxidant enzyme ( SOD, CAT and GSH ‐ Px ) ( p < .05). Meanwhile, yeast culture can reduce the accumulation of liver glycogen and improve the disorder of liver glucose metabolism caused by CPC. Furthermore, compared with CPC group, CPCY group significantly up‐regulated the expression of ZO ‐ 1, Claudin and Occludin and the activities of antioxidant enzymes (SOD, CAT) in intestine ( p < .05) as well as down‐regulated diamine oxidase (DAO) activity, D‐lactate (D‐lac) and lipopolysaccharide (LPS) contents in serum ( p < .05). In addition, the abundance of probiotics ( Lactobacillus ) increased significantly, and the abundance of intestinal potential pathogenic bacteria ( Vibrio, Brevundimonas ) decreased in CPCY group ( p < .05). In conclusion, yeast culture can alleviate the adverse effects of concentrated cottonseed protein on the growth of largemouth bass and might be used as an effective probiotic for farmed fish.