Photoelectrochemical biosensor for lead ion determination based on complementary strand aptamers. Issue 11 (3rd August 2021)

• Record Type: Journal Article
• Authors: Niu, Yanyan; Chen, Yuxue; Zhang, Xiaoping; Xie, Hui; Luo, Guiling; Sun, Wei
• Is Part Of: Journal of the Chinese Chemical Society. Volume 68:Issue 11(2021)
• Page Start: 2194
• Page End: 2201
• Publication Date: 2021-08-03
• Subjects: aptamer -- biosensor -- complementary strands -- lead ion -- photoelectrochemistry; Chemistry -- Periodicals; Electronic journals; 540.5
• DOI: 10.1002/jccs.202100142
• Languages: English
• ISSNs: 0009-4536

Abstract

Abstract: Lead ion causes heavy metal pollution, which can cause serious damage to the ecological environment and human health. In this paper, a simple and sensitive photoelectrochemical method was designed for lead ion detection by using ZnO nanorods as the photoactive substrate and complementary strand aptamers as target recognition element. ZnO nanorods with favorable stability and photoelectric activity can provide a biocompatible microenvironment for aptamer loading. In the presence of lead ion, it would combine with the recognition aptamer S2 by competing with the aptamer S1, which led to changes in surface structure and charge density before and after the recognition of target, causing regular response of photocurrent. The developed method under optimized experimental conditions presented high sensitivity and good selectivity for lead ion detection with low detection limit as 3.5 × 10 −11 mol/L. In addition, the proposed strategy was also further applied to the detection of lake water sample, which provided reliable experimental support and basis for further exploration and establishment of complex aptamer recognition and detection system. Abstract : In this paper, a simple and sensitive photoelectrochemical method was designed for lead ion detection by using ZnO nanorods as the photoactive substrate and complementary strand aptamers as target recognition element. This method provided some experimental support for further exploration and establishment of complex aptamer recognition system, as well as quantitative analysis of heavy metal ions.