Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering. (May 2015)

• Record Type: Journal Article
• Authors: Jamiolkowski, Ryan M; Kang, Sa Do; Rodriguez, AnnMarie K; Haseltine, Justin M; Galinat, Lauren J; Jantzen, Alexandra E; Carlon, Tim A; Darrabie, Marcus D; Arciniegas, Antonio J; Mantilla, Jose G; Haley, N Rebecca; Noviani, Maria; Allen, Jason D; Stabler, Thomas V; Frederiksen, James W; Alzate, Oscar; Keil, Lukas G; Liu, Siyao; Lin, Fu-Hsiung; Truskey, George A; Achneck, Hardean E
• Is Part Of: Regenerative medicine. Volume 10:Number 4(2015)
• Page Start: 447
• Page End: 460
• Publication Date: 2015-05
• Subjects: AMD3100 -- biocompatible materials -- cell Isolation -- cell therapy -- endothelial cells -- endothelial progenitor cells -- swine -- thrombosis -- tissue engineering; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme.15.2
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: Peripheral blood-derived endothelial cells (pBD-ECs) are an attractive tool for cell therapies and tissue engineering, but have been limited by their low isolation yield. We increase pBD-EC yield via administration of the chemokine receptor type 4 antagonist AMD3100, as well as via a diluted whole blood incubation (DWBI).Materials & Methods: Porcine pBD-ECs were isolated using AMD3100 and DWBI and tested for EC markers, acetylated LDL uptake, growth kinetics, metabolic activity, flow-mediated nitric oxide production and seeded onto titanium tubes implanted into vessels of pigs.Results: DWBI increased the yield of porcine pBD-ECs 6.6-fold, and AMD3100 increased the yield 4.5-fold. AMD3100-mobilized ECs were phenotypically indistinguishable from nonmobilized ECs. In porcine implants, the cells expressed endothelial nitric oxide synthase, reduced thrombin-antithrombin complex systemically and prevented thrombosis.Conclusion: Administration of AMD3100 and the DWBI method both increase pBD-EC yield.