Functional behavior and gene expression of magnetic nanoparticle-loaded primary endothelial cells for targeting vascular stents. (May 2015)

• Record Type: Journal Article
• Authors: Zohra, Fatema Tuj; Medved, Mikhail; Lazareva, Nina; Polyak, Boris
• Is Part Of: Nanomedicine. Volume 10:Number 9(2015)
• Page Start: 1391
• Page End: 1406
• Publication Date: 2015-05
• Subjects: endothelial integrity and functional potency -- gene expression -- in-stent restenosis -- magnetic cell delivery -- magnetic nanoparticles -- primary endothelial cells -- re-endothelialization; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.15.13
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To assess functional competence and gene expression of magnetic nanoparticle (MNP)-loaded primary endothelial cells (ECs) as potential cell-based therapy vectors.Materials & methods: A quantitative tube formation, nitric oxide and adhesion assays were conducted to assess functional potency of the MNP-loaded ECs. A quantitative real-time PCR was used to profile genes in both MNP-loaded at static conditions and in vitro targeted ECs.Results: Functional behavior of MNP-loaded and unloaded cells was comparable. MNPs induce expression of genes involved in EC growth and survival, while repress genes involved in coagulation.Conclusion: MNPs do not adversely affect cellular function. Gene expression indicates that targeting MNP-loaded ECs to vascular stents may potentially stimulate re-endothelialization of an implant and attenuate neointimal hyperplasia.