Nanoparticles and antigen-specific T-cell therapeutics: a comprehensive study on uptake and release. (April 2015)

• Record Type: Journal Article
• Authors: Zupke, Oliver; Distler, Eva; Jürchott, Anna; Paiphansiri, Umaporn; Dass, Martin; Thomas, Simone; Hartwig, Udo F; Theobald, Matthias; Landfester, Katharina; Mailänder, Volker; Herr, Wolfgang
• Is Part Of: Nanomedicine. Volume 10:Number 7(2015)
• Page Start: 1063
• Page End: 1076
• Publication Date: 2015-04
• Subjects: cell imaging -- cellular therapy -- drug delivery -- endocytosis -- leukemia -- nanoparticles -- NP release -- T lymphocytes -- tumor; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.14.160
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells.Materials & methods: Uptake, release and toxicity of various polymeric NP preparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-γ-ELISPOT and 51 Chromium-release assays.Results: Amino-functionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h.Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo .