Active implant combining human stem cell microtissues and growth factors for bone-regenerative nanomedicine. (March 2015)

• Record Type: Journal Article
• Authors: Schiavi, Jessica; Keller, Laetitia; Morand, David-Nicolas; Isla, Natalia De; Huck, Olivier; Lutz, Jean Christophe; Mainard, Didier; Schwinté, Pascale; Benkirane-Jessel, Nadia
• Is Part Of: Nanomedicine. Volume 10:Number 5(2015)
• Page Start: 753
• Page End: 763
• Publication Date: 2015-03
• Subjects: BMP-7 -- bone regeneration -- collagen nanofibers implant -- human mesenchymal stem cells -- microtissues -- regenerative nanomedicine; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.14.228
• Languages: English
• ISSNs: 1743-5889

Abstract

Aims: Mesenchymal stem cells (MSCs) from adult bone marrow provide an exciting and promising stem cell population for the repair of bone in skeletal diseases. Here, we describe a new generation of collagen nanofiber implant functionalized with growth factor BMP-7 nanoreservoirs and equipped with human MSC microtissues (MTs) for regenerative nanomedicine.Materials & methods: By using a 3D nanofibrous collagen membrane and by adding MTs rather than single cells, we optimize the microenvironment for cell colonization, differentiation and growth.Results & conclusion: Furthermore, in this study, we have shown that by combining BMP-7 with these MSC MTs in this double 3D environment, we further accelerate bone growth in vivo . The strategy described here should enhance the efficiency of therapeutic implants compared with current simplistic approaches used in the clinic today based on collagen implants soaked in bone morphogenic proteins.