AB0008 THE IMPACT OF STAT4 RS7574865, IL6 RS1800795, IL6R RS2228145 AND RS4845618 ON RHEUMATOID ARTHRITIS SUSCEPTIBILITY IN BELARUSIAN POPULATION. (2nd June 2020)

• Record Type: Journal Article
• Authors: Siniauskaya, E.; Kuzhir, T.; Yagur, V.; Goncharova, R.
• Is Part Of: Annals of the rheumatic diseases. Volume 79(2020)Supplement 1
• Page Start: 1307
• Page End: 1308
• Publication Date: 2020-06-02
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2020-eular.1990
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Rheumatoid arthritis (RA) is a chronic systemic disorder of the connective tissue of still unknown aetiology and complex autoimmune pathogenesis that primarily affects small joints. HLA alleles provide for 11-37% of the RA heritability, suggesting the substantial role of the non-HLA loci in genetic predisposition to RA. Among non-HLA loci, IL6, IL6R and STAT4 genes attract attention, however, the data concerning their influence on RA risk are somewhat contradictory. Objectives: The aim of the study was to analyze the involvement of four SNPs ( STAT4 rs7574865, IL6 rs1800795, IL6R rs2228145 and rs4845618) in RA susceptibility. Methods: 187 patients diagnosed with RA (mean age 58.2 ± 11.9), and 380 healthy blood donors (mean age 37.18 ± 10.69 years) were included into the study. DNA extraction from peripheral blood samples was performed using the phenol-chloroform method. SNPs were genotyped using the real-time PCR with fluorescent probes. The allele and genotype frequencies were compared using the χ2 test. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated using the VassarStats online tool. Results: Utilizing recessive genetic model we found an association between TT genotype of STAT4 rs7574865 (OR = 2.362; 95%CI [1.0378 – 5.376], p = 0.038) and RA. For IL6 rs1800795, it was found that CC genotype had significantly higher frequency among patients with rheumatoid arthritis as compared to that in controls (OR = 1.52; 95%CI [1.02 – 2.27], p = 0.0456). No associations of IL6R rs2228145 and rs4845618 SNPs with risk of RA were found in the total group of patients vs. controls. It was also shown that IL6 rs1800795 CC genotype frequency was significantly higher among the patients with RF-negative status (p = 0.0019). Conclusion: Thus, we provide evidence for association of the STAT4 rs7574865 and IL6 rs1800795 variants with risk of RA in the Belarusian population, some features of interplay being revealed between gene polymorphisms analyzed and RA antibody status. Abovementioned SNPs may contribute to RA genetic susceptibility in the Belarusian population. Disclosure of Interests: None declared