GauCNL18 mediates Verticillium wilt resistance by activating the salicylic acid immunity pathway. (December 2021)

• Record Type: Journal Article
• Authors: Li, Tinggang; Gao, Huanhuan; Tang, Xiaoning; Chen, Guangxia
• Is Part Of: Physiological and molecular plant pathology. Volume 116(2021)
• Publication Date: 2021-12
• Subjects: Verticillium wilt -- Resistance gene -- Salicylic acid signaling pathway -- Pathogenesis-related genes -- Reactive oxygen species; Plant diseases -- Periodicals; Diseased plants -- Physiology -- Periodicals; Phytopathogenic microorganisms -- Host plants -- Periodicals; 632
• DOI: 10.1016/j.pmpp.2021.101719
• Languages: English
• ISSNs: 0885-5765

Abstract

Abstract: Verticillium wilt (VW) is caused by Verticillium dahliae and significantly impacts cotton yield and quality. Breeding of resistant varieties is the most effective strategy for controlling VW. Therefore, it is important to identify VW resistance genes. The diploid wild cotton species Gossypium australe is highly resistant to VW. This study identified 33 CC-NBS-LRR (CNL) genes in the G. australe genome. GauCNL expression pattern (EP) analyses showed that EP1 cluster which included four of the 33 genes, were significantly up-regulated after V. dahliae inoculation. The EP1 cluster genes were then introduced into Arabidopsis thaliana wild type Col-0, respectively. The results showed GauCNL18 overexpression significantly increased V. dahliae resistance, indicating the functional importance of GauCNL18 . GauCNL18 promotes V. dahliae resistance by activating the salicylic acid signaling pathway to mediate up-regulating pathogenesis-related genes and reactive oxygen species accumulation. This study identified a CNL resistance gene, GauCNL18, and revealed its resistance mechanism to V. dahliae . Highlights: Verticillium wilt is caused by Verticillium dahliae and significantly impacts cotton yield and quality. The diploid wild cotton species Gossypium australe is highly resistant to Verticillium wilt. GauCNL18 overexpression significantly increased Verticillium dahliae resistance in Arabidopsis thaliana. GauCNL18 promotes Verticillium dahliae resistance by activating the salicylic acid signaling pathway to mediate up-regulated pathogenesis-related genes and reactive oxygen species accumulation.