Antimicrobial chitosan nanodroplets: new insights for ultrasound-mediated adjuvant treatment of skin infection. (June 2015)

• Record Type: Journal Article
• Authors: Banche, Giuliana; Prato, Mauro; Magnetto, Chiara; Allizond, Valeria; Giribaldi, Giuliana; Argenziano, Monica; Khadjavi, Amina; Gulino, Giulia Rossana; Finesso, Nicole; Mandras, Narcisa; Tullio, Vivian; Cavalli, Roberta; Guiot, Caterina; Cuffini, Anna Maria
• Is Part Of: Future microbiology. Volume 10:Number 6(2015:Jun.)
• Page Start: 929
• Page End: 939
• Publication Date: 2015-06
• Subjects: Candida albicans -- chitosan -- MRSA -- nanodroplet -- oxygen -- skin; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.15.27
• Languages: English
• ISSNs: 1746-0913

Abstract

ABSTRACT : Background: Chronic wounds, characterized by hypoxia, inflammation and impaired tissue remodeling, are often worsened by bacterial/fungal infections. Intriguingly, chitosan-shelled/decafluoropentane-cored oxygen-loaded nanodroplets (OLNs) have proven effective in delivering oxygen to hypoxic tissues.Aim: The present work aimed at investigating nanodroplet antimicrobial properties against methicillin-resistant Staphylococcus aureus (MRSA) or Candida albicans, toxicity on human keratinocytes (HaCaT) and ultrasound (US)-triggered transdermal delivery.Materials & methods : Nanodroplet antibacterial/antifungal properties, human cytotoxicity, and US-triggered transdermal delivery were measured through microbiological, biochemical, and sonophoresis assays, respectively.Results: OLNs and oxygen-free nanodroplets (OFNs) displayed short- or long-term cytostatic activity against MRSA or Candida albicans, respectively. OLNs were not toxic to keratinocytes, whereas OFNs slightly affected cell viability. Complementary US treatment promoted OLN transdermal delivery.Conclusion: As such, US-activated chitosan-shelled OLNs appear as promising, nonconventional and innovative tools for adjuvant treatment of infected chronic wounds.