Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis. (April 2015)

• Record Type: Journal Article
• Authors: Lai, Cheng-Kuo; Su, Jing-Chi; Lin, Yu-Hsin; Chang, Chia-Shuo; Feng, Chun-Lung; Lin, Hwai-Jeng; Lin, Chun-Jung; Tseng, Guan-Chin; Liu, Hsin-Ho; Hsieh, Jer-Tsong; Su, Hong-Lin; Lai, Chih-Ho
• Is Part Of: Future microbiology. Volume 10:Number 4(2015:Apr.)
• Page Start: 489
• Page End: 501
• Publication Date: 2015-04
• Subjects: Campylobacter jejuni -- cell cycle -- cholesterol -- cytolethal distending toxin -- DNA double-strand breaks; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.14.119
• Languages: English
• ISSNs: 1746-0913

Abstract

ABSTRACT : Aim: The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts.Materials & methods: The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse.Results: CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication.Conclusion: Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.