Polysaccharide fraction isolated from the leaves of Hordeum vulgare L. protects against colonic inflammation of systemic immune responses. (December 2021)

• Record Type: Journal Article
• Authors: Han, Hee-Soo; Kim, Seo-Yeon; Shin, Ji-Sun; Lee, Hwi-Ho; Chung, Kyung-Sook; Rhee, Young Kyoung; Cho, Chang-Won; Hong, Hee-Do; Lee, Kyung-Tae
• Is Part Of: Journal of functional foods. Volume 87(2021)
• Publication Date: 2021-12
• Subjects: Hordeum vulgare L. -- Polysaccharide -- Sepsis -- Colonic inflammation; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104765
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: BLE0 ameliorated LPS-induced endotoxemia and colonic inflammation. BLE0 exerted anti-inflammatory activities by suppressing the activation of NF-κB and AP-1 pathways. BLE0 inhibited the expression of TLR4, Myd88, IRAK1, and TRIF. Abstract: Sepsis is a life-threatening condition that can result in death accompanied by multiple organ failure. Here, we investigated the preventive effects of BLE0, a polysaccharide fraction isolated from young barley leaves ( Hordeum vulgare L.), on colonic inflammation in a mouse model of LPS-induced systemic septic shock. Pretreatment with BLE0 increased the survival rate and markedly restored colon length and crypt damage in LPS-challenged mice. BLE0 suppressed the LPS-induced expression of iNOS, COX-2, IL-6, and IL-17. The molecular mechanisms underlying the anti-inflammatory effects of BLE0 included inactivation of NF-κB and AP-1 and inhibition of monocyte/macrophage infiltration. BLE0 inhibited the mRNA expression of TLR4, Myd88, IRAK1, and TRIF induced by LPS. Collectively, BLE0 ameliorated LPS-induced endotoxemia and colonic inflammation, suggesting thatt the regulation of colonic conditions could be associated with improved systemic immune responses.