Changes in gut-brain axis parameters in adult rats of both sexes with different feeding pattern that were early nicotine-exposed. (December 2021)

• Record Type: Journal Article
• Authors: Rodrigues, V.S.T.; Moura, E.G.; Peixoto, T.C.; Soares, P.N.; Lopes, B.P.; Oliveira, E.; Manhães, A.C.; Atella, G.C.; Kluck, G.E.G.; Cabral, S.S.; Trindade, P.L.; Daleprane, J.B.; Lisboa, P.C.
• Is Part Of: Food and chemical toxicology. Volume 158(2021)
• Publication Date: 2021-12
• Subjects: Lactation -- Programming -- Nicotine -- Hyperphagia -- Gut-brain -- Dysbiosis; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112656
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Nicotine is an endocrine disruptor and imprinting factor during breastfeeding that can cause food intake imbalance in the adulthood. As nicotine affects the intestinal microbiota, altering the composition of the bacterial communities and short-chain fatty acids (SCFAs) synthesis in a sex-dependent manner, we hypothesized that nicotine could program the gut-brain axis, consequently modifying the eating pattern of adult male and female rats in a model of maternal nicotine exposure (MNE) during breastfeeding. Lactating Wistar rat dams received minipumps that release 6 mg/kg/day of nicotine (MNE group) or saline for 14 days. The progeny received standard diet from weaning until euthanasia (26 weeks of age). We measured: in vivo electrical activity of the vagus nerve; c-Fos expression in the nucleus tractus solitarius, gastrointestinal peptides receptors, intestinal brain-derived neurotrophic factor (BDNF), SCFAs and microbiota. MNE females showed hyperphagia despite normal adiposity, while MNE males had unchanged food intake, despite obesity. Adult MNE offspring showed decreased Bacteroidetes and increased Firmicutes, Actinobacteria and Proteobacteria . MNE females had lower fecal acetate while MNE males showed higher vagus nerve activity. In summary nicotine exposure through the milk induces long-term intestinal dysbiosis, which may affect eating patterns of adult offspring in a sex-dependent manner. Highlights: Early nicotine exposure affects the gut-brain axis. Maternal nicotine induces gut dysbiosis in adult progeny of both sexes. Neonatal exposure to nicotine causes vagal hyperstimulation in male rats only.