Ferulic acid-loaded nanocapsules: Evaluation of mucosal interaction, safety and antioxidant activity in human mononucleated cells. (February 2022)

• Record Type: Journal Article
• Authors: Rampelotto, Camila Reck; Pereira, Viviane Gonçalves; da Silva Silveira, Larissa; Rossato, Aline; Machado, Alencar Kolinski; Sagrillo, Michele Rorato; Gündel, André; Burger, Marilise Escobar; Schaffazick, Scheila Rezende; de Bona da Silva, Cristiane
• Is Part Of: Toxicology in vitro. Volume 78(2022)
• Publication Date: 2022-02
• Subjects: Alternative methods. Cytotoxicity. Hydroxycinnamic acid. Polymeric nanoparticles. Scavenging capacity; NC-FA ferulic acid-loaded nanocapsule suspensions -- hMNC human Mononucleated Cells -- NC-B nanocapsule suspensions without ferulic acid -- HET-CAM Hen's Egg Test – Chorioallantoic Membrane -- ABTS 2, 2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) -- H2O2 hydrogen peroxide -- ROS Reactive oxygen species.; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2021.105259
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Ferulic acid (FA) is a phenolic compound that has antioxidant, anti-inflammatory and anticarcinogenic properties besides presenting cytoprotective activity. It has limited oral bioavailability what is a challenge to its therapeutic application. In this way, this investigation aimed to develop FA-loaded nanocapsule suspensions (NC-FA) prepared with ethylcellulose and evaluate their in vitro release profile, mucoadhesion and irritation potential; scavenging capacity, cytotoxicity, cytoprotection and genoprotection against hydrogen peroxide-induced damage in hMNC (human Mononucleated Cells) culture. The nanocapsules presented physicochemical characteristics compatible with colloidal systems (NC-FA: 112 ± 3 nm; NC-B (without FA): 107 ± 3 nm; PdI < 0.2; Span<2.0 and negative zeta potential). In addition, the nanoparticulate system promoted the FA controlled release, increasing the half-life twice through the in vitro dialysis method. NC-FA and NC-B were able to interact with mucin, which is an indicative of mucoadhesive properties and the association of FA with nanocapsules showed decreased irritation by HET-CAM method. Besides, the NC-FA did not present cytotoxicity in hMNC and improved the ATBS radical scavenging capacity. Besides, it prevented, treated and reversed oxidative conditions in a H2 O2 -induced model in hMNC. Thus, this nanocarrier formulation is promising to perform more preclinical investigations focusing on diseases involving oxidative mechanisms. Highlights: Was outlined ferulic acid loaded-nanocapsule suspensions ethylcellulose based (NC-FA). The nanoparticulates were able to promote ferulic acid controlled release, as well as improved its scavenger. NC-FA demonstrated mucoadhesive properties in vitro and reduced mucosal irritation of ferulic acid (HET-CAM). NC-FA was cytocompatible with human mononucleated cells (hMNC) in vitro . NC-FA was cytoprotective and genoprotective against inducing cell damage with hydrogen peroxide (H2 O2 ) in hMNC culture.