13C metabolic flux analysis in cell line and bioprocess development. (December 2021)

• Record Type: Journal Article
• Authors: Sacco, Sarah A; Young, Jamey D
• Is Part Of: Current opinion in chemical engineering. Volume 34(2021)
• Publication Date: 2021-12
• Subjects: Chemical engineering -- Periodicals; Chemical engineering; Periodicals; 660.05
• DOI: 10.1016/j.coche.2021.100718
• Languages: English
• ISSNs: 2211-3398

Abstract

Abstract : With an ever-increasing demand for biopharmaceuticals, there is a growing need for high-producing industrial cell lines. 13 C metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks. The characterization of production cell lines altered via metabolic engineering or media optimization can help to identify desirable metabolic phenotypes. Coupled with the advent of high-throughput mini bioreactor systems and expanded metabolic modeling capabilities, 13 C flux analysis can provide an even more complete picture of intracellular metabolism. The resulting knowledge of desirable cellular traits and metabolic regulatory nodes offers new opportunities for the systematic identification of engineering targets to develop optimized cell lines and bioprocesses.