Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo. (2021)

• Record Type: Journal Article
• Authors: Dai, Yu-Lin; Jiang, Yun-Fei; Lu, Yu-An; Yu, Jiang-Bo; Kang, Min-Cheol; Jeon, You-Jin
• Is Part Of: Toxicology reports. Volume 8(2021)
• Page Start: 349
• Page End: 358
• Publication Date: 2021
• Subjects: Fx Fucoxanthin -- FxRF Fucoxanthin-rich fraction -- PM Particulate matter -- PGE Prostaglandin E -- COX Cyclooxygenase -- IL Interleukin -- NO Nitric oxide -- TNF-α Tumor necrosis factor-α -- iNOS Inducible nitric oxide synthases -- DMEM Dulbecco's Modified Eagle's Medium -- PI Propidium iodide -- DCFH-DA 2, 7-dichlorofluorescein diacetate -- H-PM Culture medium of PM-induced keratinocytes -- SE Standard error -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; Fucoxanthin -- Sargassum fusiformis -- Particulate matter -- Anti-inflammatory response; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2021.02.005
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: Particulate matter (PM) contributes to air pollution and primarily originates from unregulated industrial emissions and seasonal natural dust emissions. Fucoxanthin (Fx) is a marine natural pigment from brown macroalgae that has been shown to have various beneficial effects on health. However, the effects of Fx on PM-induced toxicities in cells and animals have not been assessed. In this study, we investigated the anti-inflammatory potential of the Fx-rich fraction (FxRF) of Sargassum fusiformis against PM-mediated inflammatory responses. The FxRF composition was analyzed by rapid-resolution liquid chromatography mass spectrometry. Fx and other main pigments were identified. FxRF attenuated the production of inflammatory components, including prostaglandin E2 (PGE2 ), cyclooxygenase-2, interleukin (IL)-1β, and IL-6 from PM-exposed HaCaT keratinocytes. PM exposure also reduced the levels of nitric oxide (NO), tumor necrosis factor-α, inducible nitric oxide synthase (iNOS), and PGE2 in PM-exposed RAW264.7 macrophages. Additionally, the culture medium from PM-exposed HaCaT cells induced upregulation of NO, iNOS, PGE2, and pro-inflammatory cytokines in RAW264.7 macrophages. FxRF also significantly decreased the expression levels of factors involved in inflammatory responses, such as NO, reactive oxygen species, and cell death, in PM-exposed zebrafish embryos. These results demonstrated the potential protective effects of FxRF against PM-induced inflammation both in vitro and in a zebrafish model.