Egg white protein polymer: an affinity matrix for protease enrichment and isolation. Issue 1 (2nd January 2022)

• Record Type: Journal Article
• Authors: Menfaatli, Esra; Zihnioğlu, Figen
• Is Part Of: Separation science and technology. Volume 57:Issue 1(2022)
• Page Start: 103
• Page End: 111
• Publication Date: 2022-01-02
• Subjects: Protease enrichment -- protease removal -- affinity purification -- affinity enrichment -- biopolymers -- egg white protein; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2021.1887235
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE results and diffusion agar plate tests supported the protease-binding efficiency of the polymer. In conclusion, egg white protein polymer can specifically bind proteases and it is a convenient and cost-effective material for enrichment, isolation, and removal of proteases.