Comprehensive analysis of LIM gene family in wheat reveals the involvement of TaLIM2 in pollen development. (January 2022)

• Record Type: Journal Article
• Authors: Yang, Xuetong; Bu, Yaning; Niu, Fuqiang; Cun, Yujie; Zhang, Lingli; Song, Xiyue
• Is Part Of: Plant science. Volume 314(2022)
• Publication Date: 2022-01
• Subjects: Bn Binucleate stage -- BSMV Barley stripe mosaic virus -- CaMV Cauliflower mosaic virus -- En Early uninucleate -- GFP Green fluorescence protein -- Lun Late uninucleate stage -- qRT-PCR Quantitative real-time polymerase chain reaction -- RNA-seq RNA sequencing -- RFP Red fluorescence protein -- Tn Trinucleate stage -- TGMS Thermo-sensitive genic male sterility -- VIGS Virus-induced gene silencing; Genome-wide analysis -- LIM genes -- Wheat -- Male sterility -- Pollen development; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.111101
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Totally, 29 LIM genes were identified in Triticum aestivum L. Each subfamily had similar gene structure which suggested that TaLIM genes were relatively conservative in the course of evolution. Phenotypic and cytological of BSMV: TaLIM2 testified that TaLIM2 was closely related to fertility conversion. Abstract: LIM domain proteins were involved in organizing the cytoskeleton, adjusting the metabolism and gene expression, some of them were specific express in pollen. LIM gene family in plants were studied in sunflower, tobacco, foxtail millet, rape, rice and Arabidopsis thaliana, however, it has not been investigated in wheat to date. In the present study, we totally characterized 29 TaLIM genes through genome-wide analysis, which were divided into two categories and five subclasses according to phylogenetic analysis. RNA-Seq analysis indicated the expression patterns of TaLIM genes have specific temporal and spatial characteristics, especially TaLIM2 was highly expressed in fertility anthers. Phenotypic and cytological of BSMV: TaLIM2 showed that it had defects in the later stage of pollen development and germination, which further testified that TaLIM2 was closely related to fertility conversion. These findings will be useful for functional analysis of LIM genes in wheat fertility and contribute to hybrid wheat breeding.