Osteogenic differentiation of human mesenchymal stem cells on substituted calcium phosphate/chitosan composite scaffold. (1st February 2022)

• Record Type: Journal Article
• Authors: Ressler, Antonia; Antunović, Maja; Teruel-Biosca, Laura; Ferrer, Gloria Gallego; Babić, Slaven; Urlić, Inga; Ivanković, Marica; Ivanković, Hrvoje
• Is Part Of: Carbohydrate polymers. Volume 277(2022)
• Publication Date: 2022-02-01
• Subjects: Chitosan -- Hydroxyapatite -- Osteogenic differentiation -- Perfusion-bioreactor -- Scaffolds -- Ionic substitution; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118883
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Ionic substitutions are a promising strategy to enhance the biological performance of calcium phosphates (CaP) and composite materials for bone tissue engineering applications. However, systematic studies have not been performed on multi-substituted organic/inorganic scaffolds. In this work, highly porous composite scaffolds based on CaPs substituted with Sr 2+, Mg 2+, Zn 2+ and SeO3 2− ions, and chitosan have been prepared by freeze-gelation technique. The scaffolds have shown highly porous structure, with very well interconnected pores and homogeneously dispersed CaPs, and high stability during 28 days in the degradation medium. Osteogenic potential of human mesenchymal stem cells seeded on scaffolds has been determined by histological, immunohistochemical and RT-qPCR analysis of cultured cells in static and dynamic conditions. Results indicated that ionic substitutions have a beneficial effect on cells and tissues. The scaffolds with multi-substituted CaPs have shown increased expression of osteogenesis related markers and increased phosphate deposits, compared to the scaffolds with non-substituted CaPs. Graphical abstract: Unlabelled Image