Network pharmacology combined with metabolomics to investigate the anti-hyperlipidemia mechanism of a novel combination. (December 2021)

• Record Type: Journal Article
• Authors: Wang, Furong; Wang, Juan; Cai, Hongdie; Yuan, Lixia; Sun, Chonglu; Peng, Xin; Yan, Weiwei; Zhang, Jiale
• Is Part Of: Journal of functional foods. Volume 87(2021)
• Publication Date: 2021-12
• Subjects: Lindera aggregate leave -- Aloe vera -- Hyperlipidemia -- Network pharmacology -- Metabolomics; AA Arachidonic acid -- AB Aloe barbadensis Miller -- ACAT Acyl–coenzyme A: cholesterol acyltransferase -- ALT Alanine aminotransferase -- APOA1 Apolipoprotein A1 -- APOB Apolipoprotein B -- AqLL-A aqueous extract of Lindera aggregate leaves and Aloe vera -- CMC-Na sodium carboxymethyl cellulose -- CVD Cardiovascular diseases -- CYP7A1 Cholesterol 7α-hydroxylase -- DHA Docosahexaenoic acid -- DL drug likeness -- DPA Docosapentaenoic acid -- GLU Glucose -- GO Gene Ontology -- HDL High-density lipoprotein -- HDL-C high-density lipoprotein cholesterol -- HFD High-fat-diet -- HLP Hyperlipidemia -- HMGCR 3-Hydroxy-3-methylglutaryl CoA reductase -- HPLC high-performance liquid chromatography -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LDL Low-density lipoprotein -- LDL-C low-density lipoprotein cholesterol -- LLA leaves of L. aggregate -- ND Normal diet -- N-HDL Non-high-density lipoprotein -- OB oral bioavailability -- PCA principal components analysis -- PPI protein-protein interaction -- PUFAS Polyunsaturated fatty acids -- SMT simvastatin -- TC Total cholesterol -- TG Triglyceride; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104848
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Lindera aggregate leaves and Aloe barborescens are both new food resources and traditional medicine. Aqueous extract of Lindera aggregate and Aloe barborescens alleviated hyperlipidemia. Network pharmacology coupled with Metabolomics decipher the multi-target mechanisms. Differential expressions of CYP7A1, ACAT and HMGCR contribute to the hypolipidemic mechanism of AqLL-A. Abstract: This study aimed to investigate the hypolipidemic effect and mechanism of aqueous extract of Lindera aggregate leaves and Aloe vera (AqLL-A) in mice. Chemical profile of AqLL-A, serum/hepatic lipid profiles, and fecal TG were measured. The major components of AqLL-A related to hyperlipidemia, quercitrin and aloin were confirmed by HPLC. AqLL-A reduced serum/hepatic lipids, while elevated serum HDL, APOA1, fecal TG levels in hyperlipidemia mice. Furthermore, 20 potential biomarkers were detected by UPLC-QTOF/MS, and all of them were approached to a normal-like level after treatment of AqLL-A. Network pharmacology combined with metabolomics elucidate the related pathways involved in hyperlipidemia. The experimental validation indicated the differential expressions of CYP7A1, ACAT and HMGCR contribute to the hypolipidemic mechanism of AqLL-A. These results suggested that AqLL-A alleviated hyperlipidemia effectively, it might play synergistic therapeutic efficacies through regulating metabolic profiles and related signaling pathway.