Anti-osteoporotic potential of Lactobacillus plantarum AR237 and AR495 in ovariectomized mice. (December 2021)

• Record Type: Journal Article
• Authors: Yu, Jing; Xia, Yongjun; Wang, Guangqiang; Xiong, Zhiqiang; Zhang, Hui; Lai, Phoency F.-H.; Song, Xin; Ai, Lianzhong
• Is Part Of: Journal of functional foods. Volume 87(2021)
• Publication Date: 2021-12
• Subjects: Osteoporosis -- Lactobacillus plantarum -- Intestinal microbiota -- Pro-osteoclastogenic signaling -- SCFA-producing bacteria; L. plantarum Lactobacillus plantarum -- E. xylanophilum group Eubacterium xylanophilum group -- ovx ovariectomized -- ZO-1 zonula occludens-1 -- JAM-3 junctional adhesion molecule 3 -- NF-κB nuclear factor kappa B -- RANK receptor activator for NF-κB -- RANKL RANK ligand -- OPG osteoprotegerin -- IL interleukin -- ROR retinoic acid receptor–related orphan receptor -- TLR Toll-like receptor -- MyD88 myeloid differentiation factor 88 -- TNFα tumor necrosis factor α -- SCFA short-chain fatty acids -- OCN osteocalcin -- DPD deoxypyridinoline -- μCT micro-computed tomography -- FITC Fluorescein Isothiocyanate -- LDA Linear Discriminant Analysis -- LEfSe LDA Effect Size -- BV/TV trabecular bone volume to tissue volume ratio -- BMD bone mineral density -- Tb.Sp trabecular space -- Tb.N trabecular number -- Tb.Th trabecular thickness -- AJac abundance-weighted Jaccard -- ANOSIM analysis of similarities; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104762
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Osteoporosis-related fracture causes morbidity and mortality. Intestinal health has been linked to osteoporosis prevention. Probiotics improved intestinal flora and exerted anti-osteoporotic effects. Probiotics modulated ovariectomy-induced osteoporosis with strain differences. Abstract: Osteoporosis is a common skeletal disease in which bone resorption exceeds bone formation. Intestinal health has been linked to reduced osteoporosis. The aim of this study was to compare the anti-osteoporotic and intestinal health effects of two strains of Lactobacillus plantarum (AR237 and AR495) in ovariectomized (ovx) C57BL/6 mice. The results showed that AR495 significantly inhibited ovx-induced bone loss, reduced intestinal permeability, increased the expression of tight junction proteins, claudin-family proteins, zonula occludens-1 (ZO-1), and junctional adhesion molecule 3 (JAM-3) in ovx mice. Furthermore, AR495 inhibited bone resorption by modulating the receptor activator for nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) system, and ameliorated ovx-induced intestinal inflammatory responses. AR495 improved intestinal microbial stability, and increased the abundance of short-chain fatty acids (SCFA)-producing bacteria and fecal SCFA content of ovx mice. Overall, the findings of this study showed that AR495 was more effective than AR237 at treating osteoporosis.