Antibacterial porous sponge fabricated with capric acid-grafted chitosan and oxidized dextran as a novel hemostatic dressing. (1st February 2022)

• Record Type: Journal Article
• Authors: Wang, Xiaoyu; Dang, Qifeng; Liu, Chengsheng; Chang, Guozhu; Song, Hao; Xu, Qing; Ma, Yue; Li, Boyuan; Zhang, Bonian; Cha, Dongsu
• Is Part Of: Carbohydrate polymers. Volume 277(2022)
• Publication Date: 2022-02-01
• Subjects: Chitosan -- Capric acid -- Oxidized dextran -- Hemostatic sponge -- Antibacterial activity; CS chitosan -- DD deacetylation degree -- MW molecular weight -- DX dextran -- OD oxidized DX -- CA capric acid -- CSCA CS modified by CA -- C-OD sponge fabricated with CSCA and OD -- EDC 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride -- NHS N-hydroxy succinimide -- HAc acetic acid -- PBS phosphate buffer saline -- FBS fetal bovine serum -- SBF simulated body fluid -- DMEM Dulbecco's modified Eagle's medium -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- DW deionized water -- NS normal saline -- WB whole blood -- HHT hydroxylamine hydrochloride titration -- WVTR water vapor transmission rate -- HSF human skin fibroblasts -- SD Sprague-Dawley -- EMP elemental mass percentage -- GD grafting degree -- PRP platelet-rich plasma -- PS pig skin; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118782
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: This work aims to fabricate multifunctional hemostatic sponges (C-ODs). Porous C-ODs were first constructed by using capric acid-modified chitosan (CSCA) and oxidized dextrans (ODs) with different oxidation degrees. Batches of experiments showed that (i) CSCA (33.39% of grafting degree), ODs, and C-ODs (100–200 μm in pore size) were synthesized, evidenced by FT-IR, 1 H NMR, elemental analysis, hydroxylamine hydrochloride titration, and SEM results; (ii) among C-ODs, C-OD2 had appropriate porosity (85.0%), swelling (20 times its dry weight), absorption, water retention, water vapor transmission, and mechanical properties; (iii) C-OD2 possessed low toxicity (relative cell viability > 86%), low hemolysis rate (0.65%), suitable tissue adhesion (4.74 kPa), and strong antibacterial efficacy (five strains); and (iv) C-OD2 's dynamic blood clotting was within 30 s. In three animal injury models, C-OD2 's hemostasis time and blood loss were fairly lower than commercial gelatin sponge. Totally, C-OD2 might serve as an ideal hemostatic dressing. Graphical abstract: Unlabelled Image