Capsicum SIZ1 contributes to ABA-induced SUMOylation in pepper. (January 2022)

• Record Type: Journal Article
• Authors: Lei, Shikang; Wang, Qingzhu; Chen, Yang; Song, Yu; Zheng, Min; Hsu, Yi-Feng
• Is Part Of: Plant science. Volume 314(2022)
• Publication Date: 2022-01
• Subjects: SUMO Small Ubiquitin-Related Modifier -- ABA Abscisic Acid -- ABI5 ABA-Insensitive 5 -- RD29A Responsive to Desiccation 29 -- RAB18 RAB GTPase Homolog B18 -- AREB3 ABA-Responsive Element Binding Protein 3 -- ABA1 ABA Deficient 1 -- ABA2 ABA Deficient 2 -- AAO3 Abscisic Aldehyde Oxidase 3 -- NCED1 Nine-Cis-Epoxycarotenoid Dioxygenase 1 -- SCE1 SUMO-Conjugating Enzyme 1 -- ASP1 Arabidopsis SUMO protease 1 -- AKT1 Arabidopsis K+ Transporter 1 -- NHX1 NA+/H+ Exchanger 1 -- SOS2 Salt Overly Sensitive 2; Abscisic acid -- Capsicum annuum -- CaSIZ1 -- Salt tolerance -- SUMOylation; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2021.111099
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Ca SIZ1, a homolog of At SIZ1, is functional replacement of At SIZ1. The nucleus-localized Ca SIZ1 was able to interact with Ca ABI5. Ca SIZ1 was involved in the ABA-induced SUMOylation and salt tolerance in pepper. Abstract: Abiotic and biotic stresses are the major factors limiting plant growth. Arabidopsis E3 SUMO ligase SIZ1 plays an essential role in plant stress tolerance. Herein, we identified a SIZ/PAIS-type protein in pepper ( Capsicum annuum ), namely Ca SIZ1, which shares 60 % sequence identity with At SIZ1. The stems and flowers of pepper had a relatively higher expression of CaSIZ1 than the fruits, leaves, and roots. ABA and NaCl treatments induced CaSIZ1 . Ca SIZ1 protein was localized in the nucleus and partially rescued the dwarf and ABA-sensitive phenotypes of Atsiz1−2, suggesting the functional replacement of Ca SIZ1 with At SIZ1. We found that Ca SIZ1 interacted with Ca ABI5, and ABA promoted the accumulation of SUMO conjugates in pepper. CaSIZ1 knockdown did not only reduce ABA-induced SUMOylation, but also attenuated the salt tolerance of pepper. Overall, the results of this study suggest that Ca SIZ1 has a significant role in ABA-induced SUMOylation and stress response.