Sodium butyrate inhibits colitis-associated colorectal cancer through preventing the gut microbiota dysbiosis and reducing the expression of NLRP3 and IL-1β. (December 2021)

• Record Type: Journal Article
• Authors: Liu, Huahuan; Bian, Zhongbo; Zhang, Qiuyu; Xiao, Zhipeng; Cao, Yu; Sun, Xiaodie; Qin, Yong; Mao, Lianzhi; Chu, Xinwei; Liao, Wenzhen; Zha, Longying; Sun, Suxia
• Is Part Of: Journal of functional foods. Volume 87(2021)
• Publication Date: 2021-12
• Subjects: Sodium butyrate -- AOM/DSS -- Gut microbiota -- NLRP3 -- Colorectal cancer; CRC colorectal cancer -- CAC colitis associated colorectal cancer -- NaB sodium butyrate -- SCFAs short-chain fatty acids -- DAI disease activity index -- AOM azoxymethane -- DSS dextran sulfate sodium salt -- PBS phosphate-buffered saline -- IP intraperitoneally -- LDA linear discriminant analysis -- LEfSe linear discriminant analysis effect size -- NF-κB nuclear factor kappaB -- OTUs operational taxonomic units -- PCoA principal co-ordinates analysis -- IBD inflammatory bowel disease -- HE hematoxylin and eosin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104862
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Sodium butyrate (NaB) ameliorated colitis and colitis associated cancer induced by AOM/DSS in mice. NaB attenuated colon inflammation induced by AOM/DSS through inhibiting NLRP3 inflammasome. NaB protected mice from AOM/DSS-induced CAC by improving colon inflammation and regulating gut microbiota dysbiosis. Abstract: Sodium butyrate (NaB) is a by-product of dietary fiber that has an anti-tumor effect on colorectal cancer (CRC). The aim of this study was to explore the effect of NaB on tumor and colitis using a colitis-associated colorectal cancer (CAC) model. CAC model was induced in mice by administration of azoxymethane (AOM) and dextran sulfate sodium salt (DSS). 0.1 M NaB in drinking water, or intraperitoneal injection of NaB (1 g/kg body weight) was given during the study period. NLRP3 inflammasome-related molecules were detected. The composition and taxonomy of colorectal microbiota were analyzed. NaB increased spleen index decreased by AOM/DSS. NaB attenuated tumors by reducing tumor load and tumor size in AOM/DSS-induced mice. NaB protected mice from CAC by improving colitis and inhibiting expression of NLRP3 and IL-1β. NaB regulated gut microbiota dysbiosis induced by AOM/DSS. In conclusion, NaB inhibited CAC by ameliorating the gut microbiota dysbiosis and inhibiting colitis.