A biosensor for the sensitive and specific measurement of arsenite using gold nanoparticles. (January 2022)

• Record Type: Journal Article
• Authors: Tabibi, Zahra; Massah, Jafar; Asefpour Vakilian, Keyvan
• Is Part Of: Measurement. Volume 187(2022)
• Publication Date: 2022-01
• Subjects: Arsenite concentration -- Arsenite oxidase -- Anodic current -- Reproducibility; Weights and measures -- Periodicals; Measurement -- Periodicals; Measurement; Weights and measures; Periodicals; 530.8
• DOI: 10.1016/j.measurement.2021.110281
• Languages: English
• ISSNs: 0263-2241

Abstract

Highlights: An arsenite biosensor using the co-deposition of ArOx and AuNPs on GC electrode. Usable up to three days after GC-AuNP-ArOx electrode preparation. A wide linear range between 10 1 -10 4 nM with a detection limit of 5 nM. No undesirable interference of heavy metals on the biosensor response. Abstract: An electrochemical biosensor for arsenite concentration determination is developed in this study using arsenite oxidase (ArOx). Gold nanoparticles (AuNP) were used in this study to improve the analytical characteristics of the biosensor by providing a suitable nano-environment for the enzyme activity. The enzyme and the nanoparticles were electrodeposited on the glassy carbon (GC) working electrode to prepare the GC-AuNP-ArOx electrode. The linear measurement range of the biosensor was between 10 1 and 10 4 nM, while its detection limit was obtained equal to 5 nM. The effects of enzyme concentration, pH, storage temperature, and storage time on the biosensor's performance were investigated. Results showed that an enzyme concentration of 7 μM at the physiological pH exerted the highest biosensor response (7 μA anodic current/10 nM arsenite). No undesirable interference of heavy metals was observed on the biosensor response, and the biosensor showed excellent reproducibility compared to a conventional chemosensor.