Intestinal immunomodulatory activity of indigestible glucan in mice and its utilization by intestinal bacteria in vitro. (December 2021)

• Record Type: Journal Article
• Authors: Horinouchi, Ayumu; Hirai, Hirokazu; Hirano, Rika; Kurihara, Shin; Takagi, Hiroki; Matsumoto, Kenji
• Is Part Of: Journal of functional foods. Volume 87(2021)
• Publication Date: 2021-12
• Subjects: Indigestible glucan -- Intestinal IgA -- Mucin -- Animal experiment -- Intestinal bacteria -- Prebiotics; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104759
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Indigestible glucan (IG) is a newly developed water-soluble glucose polymer. IG are separated into degree of polymerization ≥ 5 (High-IG) and ≤ 5 (Low-IG) fractions. High-IG induces intestinal IgA and mucin production in mice. Low-IG is utilized preferentially by Bifidobacterium strains in vitro . High-IG and Low-IG do not increase cecal short-chain fatty acids. Abstract: The functional properties of indigestible glucan (IG), a newly developed water-soluble glucose polymer, on intestinal health were investigated. Of high amylose cornstarch, fructooligosaccharides, and IG, IG showed the strongest intestinal IgA induction during the 5-week experiment with BALB/cA mice (3% per diet). Next, separated IG samples, degree of polymerization (DP) ≥ 5 (High-IG) and DP ≤ 5 (Low-IG) fractions, were fed to BALB/cA mice for 9 weeks (3% per diet). High-IG consumption immediately increased the fecal IgA, whereas Low-IG consumption gradually increased it. Fecal mucin was significantly increased in the High-IG group but not in the Low-IG group. To identify utilization of High-IG and Low-IG by intestinal bacteria, in vitro assays were performed using 57 human gut bacterial strains. Few bacteria utilized High-IG, whereas more bacteria, particularly Bifidobacterium strains, utilized Low-IG. These results indicate that IG ingestion benefits the intestinal environment via strengthening the intestinal barrier function and prebiotic effects.