Rational formulation design of injectable thermosensitive chitosan-based hydrogels for cell encapsulation and delivery. (1st February 2022)

• Record Type: Journal Article
• Authors: Dang, Phuong Anh; Palomino-Durand, Carla; Elsafi Mabrouk, Mohamed; Marquaille, Pierre; Odier, Clément; Norvez, Sophie; Pauthe, Emmanuel; Corté, Laurent
• Is Part Of: Carbohydrate polymers. Volume 277(2022)
• Publication Date: 2022-02-01
• Subjects: Thermosensitive hydrogel -- Chitosan -- Phosphate salt -- Injectable vehicle -- Cell encapsulation -- Cell delivery; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118836
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: This work reports a rational design of injectable thermosensitive chitosan systems for cell encapsulation and delivery. Using mixtures of two phosphate salts, beta-glycerophosphate and ammonium hydrogen phosphate, we demonstrate that the pH and the osmolarity can be adjusted separately by varying the molar ratios between the salts and the d -glucosamine monomers. We found the existence of a critical temperature above which gelation time decays following a power-law. This gelation kinetics can be finely tuned through the pH and salt-glucosamine ratios. Formulations having physiological pH and osmolarity were produced for chitosan concentrations ranging from 0.4 to 0.9 wt%. They remain liquid for more than 2 h at 20 °C and form a macroporous gel within 2 min at 37 °C. In vitro encapsulation of pre-osteoblastic cells and gingival fibroblasts showed homogeneous cell distribution and good cell viability up to 24 h. Such an approach provides a valuable platform to design thermosensitive cell-laden systems. Graphical abstract: Unlabelled Image