Characterization of a sulfated fucan-specific carbohydrate-binding module: A promising tool for investigating sulfated fucans. (1st February 2022)

• Record Type: Journal Article
• Authors: Mei, Xuanwei; Chang, Yaoguang; Shen, Jingjing; Zhang, Yuying; Chen, Guangning; Liu, Yanyan; Xue, Changhu
• Is Part Of: Carbohydrate polymers. Volume 277(2022)
• Publication Date: 2022-02-01
• Subjects: Sulfated fucans -- Carbohydrate-binding module -- Fluorescent probe -- Sea cucumber -- In situ visualization; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118748
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Sulfated fucans are important polysaccharides with diverse biological and biomedical activities. Carbohydrate-binding modules (CBMs) could serve as beneficial tools for the investigation of polysaccharides. Nevertheless, no sulfated fucan-binding CBM has been hitherto discovered. In the present study, a novel CBM47 domain was cloned from the marine bacterium Wenyingzhuangia fucanilytica, and heterologously expressed in Escherichia coli . The expressed protein WfCBM47 exhibited a specific binding capacity to sulfated fucans with the backbone composed of 1, 3-α-l -fucopyranose residues. Furthermore, a fluorescent probe was successfully constructed by fusing WfCBM47 with a green fluorescent protein, based on which the in situ visualization of sulfated fucan in the sea cucumber ( Apostichopus japonicus ) body wall was implemented for the first time. The discovery of WfCBM47 provided a promising tool for future investigations on sulfated fucans.