Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents. (2021)

• Record Type: Journal Article
• Authors: Singh, Deepika; Chaudhary, Deepak; Kumar, Vikas; Verma, Amita
• Is Part Of: Toxicology reports. Volume 8(2021)
• Page Start: 636
• Page End: 645
• Publication Date: 2021
• Subjects: NPs nanoparticles -- CCAgNPs silver nanoparticles of carissa carandas aqueous extract -- DEN diethylnitrosamine -- DMN dimethylnitrosamine -- AgNPs silver nanoparticles -- CC carissa carandas -- AgNO3 silver nitrate -- ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid -- DMSO dimethyl sulphoxide -- IAEC institutional animal ethical committee -- GSH glutathione -- CDNB 1-chloro-2, 4-dinitrobenzene -- FE-SEM field emission scanning electron microscopy -- DLS dynamic light scattering -- GGT γ-glutamyl transpeptidase activity -- SOD superoxide dismutase -- GPx glutathione peroxidase -- GST glutathione –S- Transferase -- GR glutathione reductase activity -- H2O2 hydrogen peroxide -- GGT gamma glutamyl transpeptidase -- XO xanthine oxidase -- LDH lactate dehydrogenase -- BUN blood urea nitrogen -- ODC ornithine decarboxylase -- ROS reactive oxygen species -- MDA malondialdehyde; Carissa carandas embedded silver nanoparticles -- NF-κB pathway -- Renal carcinoma; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2021.03.014
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Phytofabricated silver nanoparticles (CCAgNPs) were biosynthesized and characterized. CCAgNPs were evaluated against diethylnitrosamine induced renal cancer. Silver nanoparticles have an antioxidant property. Silver nanoparticles unveiled a therapeutic effect against renal cancer in vivo . Abstract: Introduction: Inflammation and oxidative stress are the main factors ascribed with interruption in the process of renal tissue impairment. The toxicity of different types of nitrosamine is well recognized in animals and humans. Administration of the smallest quantities of diethylnitrosamine or dimethylnitrosamine either orally or parenterally results into renal damage. Therapeutic effects of phytofabricated silver nanoparticles of Carissa carandas aqueous extract has been scrutinised in current study for the assessment of renal cancer activity in animal model. Methodology: Phytofabricated silver nanoparticles were characterized by using different instrumentation. Nephroprotective activity of silver nanoparticles at different doses was evaluated against N-diethylnitrosamine (200 mg/kg b.w., intraperitoneal) in animal model. Serum and renal homogenate were taken to evaluate the renal toxicity markers, oxidative stress, and antioxidant parameter, proinflammatory cytokines and histopathological study. Result: Significant outcomes of silver nanoparticles in dose dependent manner down regulated the elevated serum marker, tumour marker enzymes and histopathology observation of repaired tissue assured the renal cancer activity in animals. In addition, profile of enzymatic and non-enzymatic antioxidant, proinflammatory cytokines and tumour promotion marker also favours the anticancer property of silver nanoparticles. Conclusion: The data of current study reveals silver nanoparticles ameliorates renal oxidative stress and carcinogenesis which was induced by N-diethylnitrosamine and accredited to antioxidant and anticancer activities of phytofabricated nanoparticles by biological approach.