Prevalence and characterization of Clostridium perfringens isolated from different chicken farms in China. (December 2021)

• Record Type: Journal Article
• Authors: Xu, Wenping; Wang, Hairong; Liu, Lixue; Miao, Zengmin; Huo, Yangyang; Zhong, Zhaobing
• Is Part Of: Anaerobe. Volume 72(2021)
• Publication Date: 2021-12
• Subjects: Clostridium perfringens -- Chicken -- Antimicrobial resistance -- Multilocus sequence typing; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2021.102467
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Clostridium perfringens ( C. perfringens ) is a common pathogenic microorganism present in nature, which can cause animal and human diseases, such as necrotizing enteritis (NE) in poultry. Little is known about the current prevalence status of C. perfringens from poultry farms of different types and regions in China. From December 2018 to August 2019, we investigated the prevalence, genotype distribution and drug resistance of C. perfringens from Guangdong, Pingyin, Tai'an and Weifang. A total of 622 samples were collected and processed for C. perfringens isolation, among which 239 (38.42%) samples were determined to be positive for C. perfringens . A total of 312 isolates of C. perfringens were recovered (1–5 strains were isolated for each positive sample), and 98.72% of the isolates were identified as type A, while the others were type F. Antimicrobial susceptibility testing revealed that 47.71% of the isolates were resistant to at least five classes of commonly used antibiotics. Multilocus sequence typing (MLST) showed that 74 representative isolates were divided into 63 sequence types (STs), and the Simpson's diversity index (Ds) of the STs for the five farms was 0.9799. 37.84% of the isolates were classified into seven clonal complexes (CC1–CC7), and the isolates from the same farm were more concentrated in the minimum spanning tree. In addition, some cloaca isolates and feed isolates were distributed in the same ST or CC; this result indicates that the C. perfringens in chicken can come from the environment (feed etc.). Highlights: The prevalence of Clostridium perfringens in five different types of farms in china was investigated, 38.42% of samples were determined to be positive for C. perfringens . High diversity of ST was found among the isolates from different farms, but the isolates from the same farm were usually more close and concentrated in the minimum spanning tree. A total of 47.71% of the isolates were resistant to at least five classes of commonly used antibiotics, indicating potential public health risks.