Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks. (1st February 2022)

• Record Type: Journal Article
• Authors: Sakai, Shinji; Kotani, Takashi; Harada, Ryohei; Goto, Ryota; Morita, Takahiro; Bouissil, Soukaina; Dubessay, Pascal; Pierre, Guillaume; Michaud, Philippe; El Boutachfaiti, Redouan; Nakahata, Masaki; Kojima, Masaru; Petit, Emmanuel; Delattre, Cédric
• Is Part Of: Carbohydrate polymers. Volume 277(2022)
• Publication Date: 2022-02-01
• Subjects: Polyglucuronic acid -- Bioprinting -- 3D-printing -- Horseradish peroxidase -- Tissue engineering; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118820
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting.