Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry. Issue 2 (20th December 2021)

• Record Type: Journal Article
• Authors: Han, Di; Yu, Zhongjie; Zhang, Hong; Liu, Haipeng; Wang, Bin; Qian, Donmeng
• Is Part Of: Bioengineered. Volume 12:Issue 2(2021)
• Page Start: 10891
• Page End: 10904
• Publication Date: 2021-12-20
• Subjects: Immunoglobulin hydroxysteroid 11-beta dehydrogenase-1 -- clear cell renal cell carcinoma -- immune infiltration -- prognosis -- tumor microenvironment; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2021.1994908
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the levels of immune/stromal components and the proportion of tumor-infiltrating immune cells (TIICs) were determined in 611 ccRCC samples using the ESTIMATE and CIBERSORT analytical tools. Subsequently, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was identified by univariate Cox regression analysis, protein-protein interaction (PPI) networks and clinical survival analysis to be associated with ccRCC prognosis. At the same time, the abundance of HSD11B1 increased significantly in ccRCC was verified by western blotting, RT‑qPCR and immunostaining analysis. Furthermore, Gene Set Enrichment Analysis (GSEA) and TME suggested that HSD11B1 was involved in TME immune-related status. Taken together, the results of the present study demonstrated that HSD11B1 is a potential prognostic biomarker associated with immune cell infiltration in ccRCC.